Application

Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2) antibody produced in rat may be used in: immunoblotting immunofluorescence flow cytometry

Biochem/physiol Actions

RNA polymerase II subunit A (RNAPII) is responsible for synthesizing messenger RNA in eukaryotes. The carboxy-terminal domain (CTD) is essential for polymerase activity. The CTD mediates the coupling of transcription and processing of the nascent RNA. It also plays roles in transcription, elongation and termination. Also, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. CTD is being dynamically modified during the transcription cycle. The phosphorylation of serine is needed for RNA capping and splicing factors recruitment.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-phospho-RNA polymerase II CTD (pSer2 ) (rat IgG2a isotype) is derived from the hybridoma 3E7C7 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide containing phospho-Ser2 of human RNA Polymerase II CTD repeat. RNA polymerase II subunit A (RNAPII), also known as POLR2A, is the largest subunit of RNA polymerase II. The POLR2A gene is mapped to human chromosome 17p13.1. This polymerase contains a carboxy-terminal domain (CTD) composed of heptapeptide repeats. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase.

Immunogen

synthetic peptide containing phospho-Ser2 of human RNA Polymerase II CTD repeat.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Monoclonal Anti-RNA polymerase II CTD (pSer2) recognizes specifically phospho-Ser2 RNAPII but not phosphor-Ser5 or phosphor-Ser7.

Storage and Stability

For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not usedwithin 12 hours.